Repo Dosen ULM

Enhancement of methanol production from synthetic gas mixture by Methylosinus sporium through covalent immobilization

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dc.contributor.author Patel, Sanjay K. S.
dc.contributor.author Selvaraj, Chandabose
dc.contributor.author Mardina, Primata
dc.contributor.author Jeong, Jae-Hoon
dc.contributor.author Kalia, Vipin C.
dc.contributor.author Kang, Yun Chan
dc.contributor.author Lee, Jung-Kul
dc.date.accessioned 2022-07-28T01:09:06Z
dc.date.available 2022-07-28T01:09:06Z
dc.date.issued 2016-06
dc.identifier.issn 0306-2619
dc.identifier.uri https://repo-dosen.ulm.ac.id//handle/123456789/24938
dc.description.abstract Both methane (CH4) and carbon dioxide (CO2) are major greenhouse gases (GHGs); hence, effective pro cesses are required for their conversion into useful products. CH4 is used by a few groups of methan otrophs to produce methanol. However, to achieve economical and sustainable CH4 reduction strategies, additional strains are needed that can exploit natural CH4 feed stocks. In this study, we eval uated methanol production by Methylosinus sporium from CH4 and synthetic gas. The optimum pH, tem perature, incubation period, substrate, reaction volume to headspace ratio, and phosphate buffer concentration were determined to be 6.8, 30 C, 24 h, 50% CH4, 1:5, and 100 mM (with 20 mM MgCl2 [a methanol dehydrogenase inhibitor]), respectively. Optimization of the production conditions and pro cess parameters significantly improved methanol production from 0.86 mM to 5.80 mM. Covalent immo bilization of M. sporium on Chitosan significantly improved the stability and reusability for up to 6 cycles of reuse under batch culture conditions. The immobilized cells utilized a synthetic gas mixture containing CH4, CO2, and hydrogen (at a ratio of 6:3:1) more efficiently than free cells, with a maximum methanol production of 6.12 mM. This is the first report of high methanol production by M. sporium covalently immobilized on a solid support from a synthetic gas mixture. Utilization of cost-effective feedstocks derived from natural resources will be an economical and environmentally friendly way to reduce the harmful effects of GHGs. en_US
dc.language.iso other en_US
dc.publisher Elsevier en_US
dc.subject Greenhouse gases en_US
dc.subject Immobilizaion en_US
dc.subject Methane en_US
dc.subject Methanol production en_US
dc.subject Methylosinus sporium en_US
dc.subject Synthetic gas mixture en_US
dc.title Enhancement of methanol production from synthetic gas mixture by Methylosinus sporium through covalent immobilization en_US
dc.type Other en_US


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