Repo Dosen ULM

Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Mathanol Production from Methane.

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dc.contributor.author Mardina, Primata
dc.contributor.author Li, Jinglin
dc.contributor.author Kim, In-Won
dc.contributor.author Lee, Jung-Kul
dc.contributor.author Selvaraj, Chandrabose
dc.date.accessioned 2022-07-28T01:06:23Z
dc.date.available 2022-07-28T01:06:23Z
dc.date.issued 2016-07
dc.identifier.issn 1738-8872
dc.identifier.uri https://repo-dosen.ulm.ac.id//handle/123456789/24925
dc.description.abstract Methanol is a versatile compound that can be biologically synthesized from methane (CH4) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH4 as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH4, pH 7, 150 rpm, 30°C, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions en_US
dc.language.iso en en_US
dc.publisher KMB (The Korean Society of Microbiology and Biotechnology) en_US
dc.subject Biocatalyst en_US
dc.subject Immobilization en_US
dc.subject methane en_US
dc.subject methanol en_US
dc.subject Methylocella tundrae en_US
dc.subject whole-cell immobilization en_US
dc.title Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Mathanol Production from Methane. en_US
dc.type Other en_US


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