Repo Dosen ULM

Rare sugar production by coupling of NADH oxidase and L-arabinitol dehydrogenase

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dc.contributor.author Gao, Hui
dc.contributor.author Kim, Tae-Su
dc.contributor.author Mardina, Primata
dc.contributor.author Zhou, Pengji
dc.contributor.author Wen, Fei
dc.contributor.author Lee, Jung-Kul
dc.date.accessioned 2022-07-28T01:02:50Z
dc.date.available 2022-07-28T01:02:50Z
dc.date.issued 2016-07
dc.identifier.issn 2046-2069
dc.identifier.uri https://repo-dosen.ulm.ac.id//handle/123456789/24908
dc.description.abstract An efficient biocatalytic cell-free system containing L-arabinitol dehydrogenase (LAD) for L-arabinitol oxidation and NADH oxidase (Nox) for cofactor regeneration was successfully constructed and used for L-rare sugar production. The recombinant LAD (HjLAD) from Hypocrea jecorina suffered from NADH inhibition. Applying Nox to the cell-free HjLAD system drove the thermodynamically unfavorable equilibrium from L-arabinitol to L-xylulose, along with the regeneration of the oxidized cofactor NAD+. Response surface methodology was used to optimize the conditions for L-xylulose production. The optimal conditions for biocatalytic production of L-xylulose were found to be 4.9 U mL 1 HjLAD, 8.2 mM NAD+ at pH 8.0, and 30.9 C; a high conversion rate of 78.4% was achieved under these conditions. We demonstrate a cell-free enzyme coupling system enabling efficient cofactor recycling and providing high yields of L-xylulose. The results indicate that this coupling system provides a new biocatalytic method for L-rare sugar production en_US
dc.language.iso en en_US
dc.publisher Royal Society of Chemistry (RSC) en_US
dc.title Rare sugar production by coupling of NADH oxidase and L-arabinitol dehydrogenase en_US
dc.type Article en_US


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