Abstract:
Abstract. Pasak bumi (Eurycoma longifolia Jack.) has various benefits, such as the root of E.
longifolia is often used as an anti-microbial, anti-malarial, anti-inflammatory, dysentery and
aphrodisiac. The antioxidant activity is closely related to the ability of E. longifolia root to
maintain a fit condition. The benefits of the medicinal plants can be felt by the general public after
going through both traditional and modern processing processes. One of the modern processing
methods is to isolate secondary metabolites in plants or known as extraction. This study aims to
determine the antioxidant activity of the roots of extracted E. longifolia using 96% ethanol solvent
with different extraction methods. Extraction was performed by maceration, percolation, reflux,
and soxhletation methods. The antioxidant activity test was carried out using the DPPH(1,1-
diphenyl-2-picrylhydrazyl) method. The results shows that the antioxidant activity of E. longifolia
extract using various extraction methods in the order soxhletation > reflux > percolation >
maceration. From this research, it can be concluded that the percolation can be developed as
extraction method of E. longifolia roots. Keywords: extraction, methods, antioxidant, Eurycoma
longifolia, root
