Abstract:
Malnutrition causes disruption of spermatogenesis in children. Pasak Bumi has the potential to
increase spermatogenesis due to malnutrition. Aimed of research is to proved Pasak Bumi as a food
supplement to improve spermatogenesis disorders due to malnutrition in malnourished rats. The
research method used analytical observational method by observing the histological preparations of
the testes of malnourished rats. Observations using a binocular microscope were analyzed with
Optilab Camera and Image Raster software. There are 5 groups of experimental: M: malnutrition
without intervention; P1: malnutrition + standard feed + PB 7.5 mg/kgBW; P2: malnutrition + standard
feed + PB 15 mg/kgBW; P3: malnutrition + standard feed + PB 22.5 mg/kgBW; P4: malnutrition +
standard feed + PB 30 mg/kgBW; Research parameters were number of seminiferous tubules, primary
spermatocytes and Leydig cells. Data analysis used the Anova One way test with a confidence level of
95%. The results showed number of seminiferous tubules, primary spermatocytes and Leydig cells in
the M group was less than the P1-P4 group. This suggests malnutrition causes disruption of
spermatogenesis. Conclusion the administration of Pasak Bumi extract at various doses increased
spermatogenesis in malnourished.
Keywords: Pasak Bumi, malnutrition, seminiferous tubules, primary spermatocytes, Leydig cells
