Kualitas semen beku kambing peranakan Boer yang dikriopreservasi dengan pengencer Tris kuning telur dan berbagai konsentrasi ekstrak daun kelor

Abstract

Moringa leaves contain various nutrients that can protect spermatozoa during semen cryopreservation. The purpose of this study was to examine the effectivity of Moringa leaf extract into egg yolk tris extender on the quality of Boer crossbreed goat frozen semen. Fresh semen was divided into four tubes in equal volume, and centrifuged with 3,000 RPM for 20 minutes. Seminal plasma was removed, and pellet (spermatozoa) was diluted with four different extenders as a treatment. The study used a completely randomized design with four treatments, namely: 73% tris-based extender + 20% egg yolk + 7% glycerol (control), 71% tris-based extender + 20% egg yolk + 7% glycerol + 2% Moringa leaf extract daun kelor (MLE2), 69% tris-based extender + 20% egg yolk + 7% glycerol + 4% Moringa leaf extract (MLE-4), and 67% trisbased extender + 20% egg yolk + 7% glycerol + 6% Moringa leaf extract (MLE-6), respectively. Diluted Rizal et al semen were loaded into mini straw (0.25 ml), equilibrated in the refrigerator at 5o C for four hours and frozen by placing the straw 10 cm above the liquid nitrogen surface for 15 minutes. Variables including percentage of spermatozoa motility, live, and intact plasma membrane (IPM) were evaluated after dilution and thawing. Results of this study showed that the addition of Moringa leaf extract into egg yolk tris extender was able to maintain the quality of Boer crossbreed goat frozen semen. The addition of 4% Moringa leaf extract was the best concentration in producing frozen semen of Boer crossbreed goat. The percentage of spermatozoa motility after thawing in the control, MLE-2, MLE-4, and MLE-6 were 41.25%, 45%, 50%, and 47.5%, respectively. In conclusion, the addition of 4% Moringa leaf extract into egg yolk tris extender is the best concentration in producing frozen semen of Boer crossbreed goat. Key words: Moringa leaf extract, semen cryopreservation; Boer crossbreed goat.