EKSTRAKSI LIPID DARI BIOMASSA Synechococcus sp. DENGAN METODE OSMOTIC SHOCK

Abstract

Lipid extraction of Synechococcus sp. biomass using osmotic shock method has been performed. The aims of this reserach are to obtain the optimum extraction conditions, lipid productivity, and lipid characteristics. In this research NaCl, KCl, CH3COONa and glucose with concentration of 50; 100; 150; 200; and 250 g/L were used as osmotic agents. The highest yield and lipid productivity were 12.19% and 95.42 mg/L/day, respectively when CH3COONa (250 g/L) was used as the osmotic agent. The characterization of lipid after esterification with GCMS obtained five main methyl ester compounds that were methyl palmitic, methyl linoleate, methyl stearate, methyl elaidic, and methyl oleate. Based on the characterization results, it is concluded that the microalgae Synechococcus sp. is potential to develope as raw material of biodiesel.