Repo Dosen ULM

Laparoscopic Procedures Impact On Mast Cell Mediators, Extracellular Matrix and Adhesion Scoring System in Rats

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dc.contributor.author Poerwosusanta, Hery
dc.date.accessioned 2021-08-02T02:48:03Z
dc.date.available 2021-08-02T02:48:03Z
dc.date.issued 2020-08-26
dc.identifier.uri https://repo-dosen.ulm.ac.id//handle/123456789/20671
dc.description.abstract Background: Laparoscopic procedures under certain pressure have the potential to cause intra-abdominal ad- hesions. However, the pathomechanism of this disorder is unknown. Release of mast cell mediators due to mast cell degranulation is thought to be the cause. Materials and methods: Thirty male Sprague-Dawley rats were grouped into five groups (n = 6 per group): one control group and four intervention groups to which 60 min insufflation was performed using carbon dioxide at 5, 8, 10 and 12 mmHg. Seven days after laparoscopy, we euthanized and evaluated the levels of histamine, tryptase, and chymase of peritoneal fluid, the thickness of ECM of peritoneal tissue, and intraabdominal adhesion scoring system. Results: Histamine and tryptase levels in peritoneal fluid were significantly higher at the 10- and 12 mm Hg intervention compared to control (histamine: 0.50 ± 0.35 vs. 0.41 ± 0.41 vs. 0.04 ± 0.02 ng/mL, respectively; and tryptase: 0.69 ± 0.11 vs. 0.65 ± 0.05 vs. 0.48 ± 0.02 ng/ml respectively). The ECM was significantly thicker in the intervention groups at 10- and 12-mm Hg compared to control (71.3 [66.7–85.2] vs. 48.4 [34.5–50.3] vs. 10.25 [8.7–12.1] μm, respectively). Moreover, the intra-abdominal scoring was also significantly higher in the intervention groups at 10- and 12 mm Hg compared to control (4 [0–4] vs. 4.5 [4–5], vs. 0, respectively). Conclusions: Laparoscopic procedures increase the release of mast cell mediators in peritoneal fluid, the thickness of ECM and intraabdominal adhesion scoring in rats, implying that it might increase the possibility of intra- bdominal adhesion in humans. en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries 58;
dc.subject Peer Review en_US
dc.title Laparoscopic Procedures Impact On Mast Cell Mediators, Extracellular Matrix and Adhesion Scoring System in Rats en_US
dc.type Article en_US


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