Hak Paten - Dayak Onions (Eleutherine Americana L.Merr) Reduced Mesothelial Detachment After Laparoscopic

Abstract

Background: Laparoscopy induces mesothelial structure changes and detachment. The studies about preventing the mesothelial cell detachment were rarely found. Dayak tribe uses the Dayak onion (Eleutherine americana L. Merr) as a wound-healing agent due to its anti-inflammatory and antioxidant activity. This study aimed to prove the anti-inflammatory and antioxidant activity of Dayak onion to prevent mesothelial cells damage after laparoscopy. Methods: Thirty males Sprague-Dawley Rattus norvegicus were divided into five groups (n = 6 per group): (a) control, (b) mediclor, (c) Dayak onion 30 mg/kg, (d) Dayak onion 60 mg/kg, and (e) Dayak onion 90 mg/kg body weight. Transforming growth factor-beta (TGF-β) and total oxidant status (TOS/H2O2) from peritoneal fluid was determined 24 h after laparoscopy. Histopathological analysis of mesothelial cell numbers in the peritoneum, small intestine, greater omentum, and liver was performed 7 days after the procedure. Results: In-silico analysis result suggested one of the Dayak onions’ active compounds, eleutherine, has a potential activity as an anti-inflammatory and modulator of TGF-β. Also, the TGF-β level, the number of mesothelial cells detachment, and ZO-1 expression were significantly decreased following Dayak onion administration (p<0.05). Conclusions: Our study showed that Dayak onion administration in rats reduced TGF-β level, number of mesothelial cells detachment, and ZO-1 expression following laparoscopy.

Keywords: Laparoscopy, mesothelial cell, inflammatory response, Dayak onion