Hak Paten - Mast Cells Degranulation Triggers Intra Abdominal Adhesion After Laparoscopic

Abstract

Abstract 51 Background: Laparoscopic at specific pressures has potential intra-abdominal adhesion. 52 Unfortunately, the pathomechanism of intra-abdominal adhesion is still challenging to 53 understand. Proving the effect of mast cell degranulation with intra-abdominal adhesion 54 was the aim of this study. 55 Methods: Thirty male Sprague-Dawley rats were grouped into five groups (n = 6 per 56 group), namely: a) the control group and b) the intervention group 5 mmHg, 8 mmHg, 10 57 mmHg, and 12 mmHg performed 60 minutes insufflation using carbon dioxide (CO2) at 5, 58 8, 10 and 12 mmHg, respectively. Seven days after laparoscopy, our study evaluated: a) the 59 number and percentage of mast cell degranulation in the peritoneum, mesentery, and 60 omentum; b) histamine, tryptase, and chymase of peritoneal fluid; c) thickness of 61 extracellular matrix peritoneal tissue and d) intra-abdominal adhesion scoring. 62 Results: There was a statistically significant higher in a) mast cell infiltration and 63 degranulation, b) histamine and tryptase levels of peritoneal fluid, c) extracellular matrix 64 thickness, and d) adhesion scoring at 10 mm Hg (p < 0.05). 65 Conclusions: Our study proved that laparoscopy results in mast cell degranulation that 66 increases in intra-abdominal adhesion. 67 68 Keywords: Laparoscopy, Mast cell infiltration and degranulation, Extracellular matrix 69 thickness, Intra-abdominal adhesion