Peer Review - Mast Cell Degranulations Trigers Intra Abdominal Adhesion After Laparoscopic

Abstract

Background: Laparoscopic at specific pressures has potential intra-abdominal adhesion. Unfortunately, the pathomechanism of intra-abdominal adhesion is still challenging to understand. Proving the effect of mast cell degranulation with intra-abdominal adhesion was the aim of this study. Methods: Thirty male Sprague-Dawley rats were grouped into five groups (n = 6 per group), namely: a) the control group and b) the intervention group 5 mmHg, 8 mmHg, 10 mmHg, and 12 mmHg performed 60 minutes insufflation using carbon dioxide (CO2) at 5, 8, 10 and 12 mmHg, respectively. Seven days after laparoscopy, our study evaluated: a) the number and percentage of mast cell degranulation in the peritoneum, mesentery, and omentum; b) histamine, tryptase, and chymase of peritoneal fluid; c) thickness of extracellular matrix peritoneal tissue and d) intra-abdominal adhesion scoring. Results: There was a statistically significant higher in a) mast cell infiltration and degranulation, b) histamine and tryptase levels of peritoneal fluid, c) extracellular matrix thickness, and d) adhesion scoring at 10 mm Hg (p < 0.05). Conclusions: Our study proved that laparoscopy results in mast cell degranulation that increases in intra-abdominal adhesion.

Keywords: Laparoscopy, Mast cell infiltration and degranulation, Extracellular matrix thickness, Intra-abdominal adhesion