Repo Dosen ULM

Peer Review - The Effect of Laparoscopy On Mast Cell Degranulation and Mesothelium Thickness in Rats

Show simple item record

dc.contributor.author Poerwosusanta, Hery
dc.date.accessioned 2021-01-19T01:17:37Z
dc.date.available 2021-01-19T01:17:37Z
dc.date.issued 2020-11-16
dc.identifier.other 000179862
dc.identifier.uri https://repo-dosen.ulm.ac.id//handle/123456789/18776
dc.description Peer Review en_US
dc.description.abstract Background: Laparoscopy induces adhesion due to ischemia-reperfusion injury. However, the detail pathomechanism is poorly understood. This study aimed to investigate the impact of laparoscopy on mast cell and mesothelium morphological changes in the rat. Methods: Forty-nine males of Sprague-Dawley Rattus norvegicus were divided into four groups: a) control and b) intervention groups P1, P2, and P3 that underwent 60 min laparoscopy using carbon dioxide (CO2) insufflation at 8, 10, and 12 mmHg groups, respectively. Serum hydrogen peroxide (H2O2), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and oxidative stress index (OSI) levels were determined 24 h after laparoscopy. At the same time, histopathological analyses of mast cell infiltration and degranulation and mesothelium thickness in the liver, greater omentum, mesenterium, small intestine, and peritoneum were performed 7 days after the procedure. Results: H2O2, MDA, and OSI levels were significantly increased in the intervention groups compared with the control (p < 0.05), while the SOD and CAT levels were decreased in the intervention groups compared with the control (p < 0.05). Mast cell infiltration and degranulation were higher in the intervention groups than in control (p < 0.05), while the mesothelium thickness was significantly lower in the laparoscopy groups than in control (p < 0.05). Interestingly, the decrease in mesothelium thickness was strongly associated with the increase in mast cell infiltration and degranulation (p < 0.01). Conclusions: Our study showed that laparoscopy in rats increased mast cell infiltration and degranulation, which also resulted and correlated with a decrease in mesothelial thickness. Keywords: Laparoscopy, Mast cell infiltration and degranulation, Mesothelium thickness, Oxidative stress en_US
dc.publisher Fakultas Kedokteran ULM en_US
dc.subject Peer Review en_US
dc.title Peer Review - The Effect of Laparoscopy On Mast Cell Degranulation and Mesothelium Thickness in Rats en_US
dc.type Other en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Browse

My Account