Anticataract Activity of Ethanolic Extract of Garcinia Mangostana Linn Pericarp on Glucose-induced Cataractogenesis in Goat Lens

Abstract

BACKGROUND: One of the main causes of blindness for diabetic patients is cataract. The molecular mechanisms that may be involved in diabetic cataract include polyol pathway flux, increased formation of advanced glycation end products, osmotic stress, and elevated oxidative stress. AIM: The aim of the study was to explore the anticataract activity of the ethanol extract from pericarp of Garcinia mangostana Linn (GML) in Goat eye lens. METHODS: Goat eye lenses separated to five groups were incubated in M199 medium, added by penicillin of 100 U/ml and streptomycin of 0.1 mg/ml in a 37°C incubator with 95% O2 and 5% CO2 for 120 h. Group I served as the normal control. Group II (toxic control) the lenses were added by 30 mM glucose. The lenses in Groups III, IV, and V nurtured in M199 medium with 30 mM of glucose added by 100, 500, and 1000 μg/ml of GML pericarp extract. Carboxymethyllysine (CML), GRP78, DDIT3, active caspase 3, and MDA levels were measured by enzyme-linked immunosorbent assay assays. Comparisons of biochemical parameters were conducted using one-way ANOVA with post hoc Tukey test. RESULTS: The administration of glucose 30 mM increased MDA, CML, DDIT3, and GRP78, and active caspase 3 levels in Group II. Groups III, IV, and V which treated with GML pericarp extract decreased MDA, CML, GRP78, and DDIT3, and active caspase 3 levels inside the lens. On lens capsule, the CML and GRP78 levels increased. CONCLUSION: Ethanol extract of GML pericarp potentially showed antioxidant, anti-glycation, DDIT3 inhibitor, and anti-apoptotic activity in Goat cultured lenses Keywords: Garcinia mangostana L; Anticataract; DDIT3 inhibitor; Anti-glycation