Artikel : The Inhibition Effect of Kelakai (Stenochlaena palustris) Extract on Cadmium-Induced Glycation and Fructation In-vitro

Abstract

The objectives of this study were to determine the inhibition effect of kelakai (Stenochlena palustris) extract against cadmium (Cd)-induced glycation and fructation in vitro. The inhibiton effect of kelakai extract was determined by assessing the concentration of methylglyoxal (MG), Advanced Oxidation Protein Products (AOPPs), and carbonyl compound (CC). In this present study, glycation and fructation reaction were made using bovine serum albumin (BSA) as a protein and glucose or fructose as a reducing sugar and Cd as a catalyst. Each model then divided into 5 groups consisting of: BSA + glucose or fructose as group 1 (T1); BSA + glucose or fructose + Cd as group 2 (T2); BSA + glucose or fructose + Cd + 5 mg/l of kelakai extract as group 3 (T3); BSA + glucose or fructose + Cd + 10 mg/l of kelakai extract as group 4 (T4); and BSA + glucose or fructose + Cd + 15 mg/l of kelakai extract as group 5 (T5). Results of this present study shows that Cd could increased the rate constant of MG, AOPPs, and CC formation both in glycation or fructation. Administration of plant extract could decreased the rate constant of MG, AOPPs, and CC formation induced by Cd both in glycation and fructation. The results also shows that the rate constant of the formation of MG, AOPPs, and CC in fructation were higher than glycation. In conclusions, the resuts of this present study indicated that Cd could increased the rate constant of MG, AOPPs, and CC formation while the administration of kelakai extract could decreased the rate constant. It suggest that kelakai extract have inhibitory effect against Cd-induced glycation and fructation reaction in vitro.