Abstract:
One of the plants that is used empirically by people in South Kalimantan as a traditional medicine is bilaran
tapah (Argyreia nervosa (Burm. F.). This plants that has purposed antiinflamation, antimicroba, antidiabetic,
diuretic, and aphrodisiac. This research aims to isolate and explore the chemical compounds of n-hexana
fraction of A. nervosa leaves. Extraction is conducted by using maceration method. Isolation is conducted by
using Vacumn Liquid Chromatography and gravity chromatography column. Identification qualitative assay of
compound used TLC test, UV-Vis and FTIR spectrophotometry. The extraction of 500 grams A. nervosa leaves
rough powder with 96% ethanol extracts produces 16,92% of rendement. The fractionation of 30 grams ethanol
extracts with n-hexane produces 23,60% of rendement. N-hexane fraction by using VLC with mobile phase nhexane- ethyl acetate (25:1; 20:1; 15:1; 10:1; 9:1; 8:2; 7:3 ; and 6:4) v/v produces fractions A, B, C, D, E, F, G,
and H. Fraction C is chosen to be isolated by using column chromatography with mobile phase n-hexane : ethyl
acetate (20:1)v/v. TLC qualitative test with ammonia vapor, dragendorff, and Liebermann-Burchard reagent
shows that C-4 isolates contains terpenoid compounds. Analysis of C-4 isolates by using UV-Vis
spectrophotometry reach peak at λ 245 nm. Analysis FTIR spectra shows that the functional groups of C-4
isolates are -OH (3309.85 cm-1), -CH aliphatic (2939.52 cm -1 and 2870.08 cm-1), C = C (1635.64 cm- 1) and
C-O (1033,85 cm-1).
Keywords : Argyreia nervosa (Burm. F.); ethanol extract; isolation; identification; n-hexane
