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ABSTRACT
Background: Periodontitis is an inflammatory disease of dental support
tissue caused by certain groups of microorganisms, resulting in
progressive destruction of the periodontal ligament and alveolar bone,
with pocket formation, recession, or both1
. Destruction of periodontal
caused by the host defence.
Objective: Granulicatella adiacens is a small Gram-positive, non-motile,
non-spore-forming, facultatively anaerobic coccus. As a member of
Nutritionally Variant Streptococci, genus Granulicatella, it is a part of
the normal microbiota in the oral cavity, urogenital, and intestinal tract.
It may also participate in periodontitis, caries, and endodontic
infections. Our aim in this study was to investigate the presence
of Granulicatella adiacens in periodontal disease
Methods: A total 18 samples from patients with chronic periodontitis
was collected, by using the conventional PCR technique for checking
the presence of bacterial bands, followed by using DNA sequence
methods, to complete the examination of bacteria with Conventional
PCR which results are used as a basis for nucleotide examination on
the NCBI Gene Bank using BLAST analysis.
Result: In this study, Granulicatella adiancense was found in 13
samples from 18 solated samples from patients with chronic
periodontitis and in periodontal pocket depth more than 7 mm.
Conclusion: Granulicatella adiancense is a normal flora bacteria in the
oral cavity and chronic periodontitis conditions, the presence of this
bacteria is detected in the deeper periodontal pocket depth.
Keywords: Chronic periodontitis, DNA squence, Granulicatella
adiacens
Correspondence:
Harun Achmad
Lecturer of Pedodontics Department, Dentistry Faculty, Hasanuddin
University
Makassar, Indonesia
E-mail: harunachmader@gmail.com
DOI: 10.31838/srp.2020.4.59
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