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Background and Objective : Ralstonia solanacearum is a bacterium that causes wilt disease in
bananas (Kepok: Lokal Indonesia), this disease is very dangerous and has spread evenly, and
already at high attack rate causing 100% loss. This bacterium has not been known for its
characteristics and its pathogenicity from South Kalimantan, Indonesia. Research purposes :
Determine R. solanacearum including biovar (strain) and pathogenicity, bacteria capability and
know the pathogenicity of isolate from banana origin in South Kalimantan. Materials and
Methods: This research was conducted by tracing bacterial isolate in carbohydrate medium and
allowed to form acid by showing the change of medium color from green to yellow as a positive
reaction of carbon source usage, and pathogenicity test by describing plants showing symptoms of
pain and counting time of incubation period. Results : That isolate of R. solanacearum bacteria
from Kota Banjarbaru, Banjar, Tapin, Tabalong and Kotabaru districts were able to use carbon
source from lactose, maltose, mannitol, dulsitol and sorbitol. R. solanacearum tested was able to
cause sick plants and showed symptoms of wilt averaging 60 days after inoculation, isolates from
Banjarbaru, Banjar, Tapin and Kotabaru, while Tabalong isolates caused wilting symptoms after an
average of 66 days. Conclusions : Isolate R. solanacearum from Banjarbaru, Banjar, Tapin,
Tabalong and Kotabaru, Indonesia were able to use carbon source from lactose, maltose, mannitol,
dulsitol and sorbitol and belong to biovar group 3, very high pathogenicity level and incubation
period average 60 -66 days after inoculation.
Key words: Ralstonia solanacearum, Biovar, Pathogenicity, Carbon source, wilt disease |
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