| dc.description |
Caryopteris incana (Verbenaceae) has been used to treat cough, arthritis, and eczema in Oriental
medicine. The two fractions (CHCl3- and BuOH fractions) and the essential oil of the plant material were
subjected to the inducible nitric oxide synthase (iNOS) assay. The IC50 of the CHCl3 fraction and the essential oil
on LPS-induced macrophage RAW 264.7 cells were 16.4 μg/mL and 23.08 μg/mL, respectively. On gas
chromatography (GC)-mass spectroscopy (MS) analysis, twenty-five components representing 85.5% amount of
total essential oil were identified. On the chromatogram, three main substances, trans-pinocarveol, cis-citral, and
pinocarvone, occupied 18.8%, 13.5% and 18.37% of total peak area. Furthermore, by HPLC-UV analysis, six
compounds including one iridoid (8-O-acetylharpagide)- and five phenylethanoid glycosides (caryopteroside,
acteoside, phlinoside A, 6-O-caffeoylphlinoside, and leucosceptoside A) isolated from the BuOH fraction were
quantified. The content of six compounds were shown as the following order: caryopteroside (162.35 mg/g) > 8-
O-acetylharpagide (93.28 mg/g) > 6-O-caffeoylphlinoside (28.15mg/g) > phlinoside (22.60mg/g) > leucosceptoside A
(16.87 mg) > acteoside (7.05 mg/g).
Keywords − Caryopteris incana, glycoside, HPLC, inducible nitric oxide synthase, Verbenaceae |
|