| dc.creator |
RIZALI, AKHMAD |
|
| dc.date.accessioned |
2020-06-15T03:59:56Z |
|
| dc.date.available |
2020-06-15T03:59:56Z |
|
| dc.identifier |
http://eprints.ulm.ac.id/3757/1/Bacillus_Thurungiensis_isolates_from_indonesia_toxic_to_IJB-Vol-12-No-3-p-154-161.pdf |
|
| dc.identifier |
RIZALI, AKHMAD Bacillus Thurungiensis isolates from indonesia toxic to mosquito insects. International Journal of Biosciences . ISSN 2220-6655 |
|
| dc.identifier.uri |
https://repo-dosen.ulm.ac.id//handle/123456789/11490 |
|
| dc.description |
B. thuringiensis has been studied worldwide over the past decades, mainly because this gram-positive bacterium
produce significant amount of crystal proteins with toxic activity against economically important insect larvae.
Most strains of B. thuringiensis produce delta-endotoxin crystals toxic to lepidopteran insects such moth. But
some strains of B. thuringiensis produce delta-endotoxin crystals toxic to dipteran insects such as mosquitoes
and blackflies. One gram of soil samples was suspended in 9ml of sterile distilled water and shaken for 5min. the
upper layer of the soil suspension was transferred to a test tube and heated at 80oC for 5min in water bath to kill
non-spore-forming organism and vegetative cells.to prepare the sporulated culture, bacteria were grown on
nutrient agar pH 7.2, at 30oC for 4 days., it was observed and photographed with (SEM). The results that serovar
entomocidus (original starin) produced bipyramidal-formed and entomocidus INA288 produced large cuboidalform
crystals. The PCR screening showed that cry4 Aa had a novel mosquitocidal cry gene and had 70 kDa
peptide, but serovar entomocidus (original strain) encodes only cry1Aa, cry1Ab, cry1B and cryIC, which have
not been thought to be dipteracidal activity.they had differences form crystal protein, and unique as insecticidal
to control the same orders (mosquitocidal) |
|
| dc.format |
text |
|
| dc.publisher |
Innspud |
|
| dc.relation |
http://www.innspub.net/ijb/bacillus-thurungiensis-isolates-indonesia-toxic-mosquito-insects/ |
|
| dc.relation |
http://eprints.ulm.ac.id/3757/ |
|
| dc.subject |
AI Indexes (General) |
|
| dc.title |
Bacillus Thurungiensis isolates from indonesia toxic to mosquito insects |
|
| dc.type |
Article |
|
| dc.type |
PeerReviewed |
|