This research was done to explain about blood profile of mudskipper(periophthalmodon schlosseri) by calculation erythrocytes count,hemoglobin,haematocrit,calculation of index erythrocyte which consist of calculation of mean corpuscular volume(MCV), mean cell hemoglobin (MCH) and mean cell hemoglobin concentration(MCHC),also lone tansect methot.This research was used P.schlosseri with length about 25,4 -28. Samples were taken from Barito river esttaury at intertidal zone of bahagia river, Tanipah billage,Aluh-Aluh South Kalimatan blood test of P.shlosseri based on hematology method.Result showed that erythrocyte count was 3,36+0,1x10 cell/ul;MCH 36,88+1,82 pg/cell;MCHC 29,80+1,18 g/dL and leukocyte count was 222,62+5,09 cell/uL.Blood profile of P.schlosseri from Brito river Estuary Souyth Kalimantan is the result od environment adaptation and amphibious behavioe(air-breathing). Keyword:blood,erythrocyte,leukocyte,mudskipper