| dc.creator |
Illiandri, Oski |
|
| dc.date.accessioned |
2020-06-15T03:57:48Z |
|
| dc.date.available |
2020-06-15T03:57:48Z |
|
| dc.identifier |
http://eprints.ulm.ac.id/1721/1/IJPCR%2CVol8%2CIssue12%2CArticle211.pdf |
|
| dc.identifier |
Illiandri, Oski Moderate Concentrations of TNF- α Induce BMP-2 Expression in Endothelial Cells. Moderate Concentrations of TNF- α Induce BMP-2 Expression in Endothelial Cells. |
|
| dc.identifier.uri |
https://repo-dosen.ulm.ac.id//handle/123456789/10248 |
|
| dc.description |
Tumor necrosis factor-alpha (TNF-α) is best-known as a potent pro-inflammatory cytokine involved in many
cardiovascular diseases. During vascular calcification, TNF-α has been reported topromote osteogenic differentiation of
human vascular smooth muscle cells (VSMC) and mesenchymal stem cells (MSCs). In contrast, there is alack of data
reporting the osteoinductive effect of TNF-α in endothelial cell. In this study, experiments were performed to investigate
and determine theoptimum dose of TNF-α that induces expression of the osteoinductive factor bone morphogenetic protein
(BMP-2) in endothelial cells. Human vein endothelial cells were treated with TNF-α at doses of 0, 2, 5, 10, or 20 ng/mL
for2, 8, or 24 h time intervals. BMP-2 cell expression was evaluated using immunocytochemistry staining by calculating
the percentage of BMP-2 positive cells. Apoptosiswas determined by counting the number ofpyknoticcells. In this study,
we found that the optimum dose of TNF-α thatinduces BMP-2 expression in endothelial cells was 5 ng/mL at the 8 h time
interval. Lower (2 ng/mL) orhigher (10 and 20 ng/mL) concentrations of TNF-α had minimal effects on BMP-2 expression.
Moreover, higher concentrations of TNF-α treatment (10 and 20 ng/ml) at8 h and 24 h increased the presence of pyknotic
endothelial cells, which representthefinal stage of apoptosis. |
|
| dc.format |
text |
|
| dc.relation |
http://ijpcr.com/volume8issue12/ |
|
| dc.relation |
http://eprints.ulm.ac.id/1721/ |
|
| dc.subject |
AI Indexes (General) |
|
| dc.title |
Moderate Concentrations of TNF- α Induce BMP-2 Expression in Endothelial Cells |
|
| dc.type |
Article |
|
| dc.type |
PeerReviewed |
|